Construction of AAV Vector

Overview

Adeno-associated virus (AAV) is an unenveloped virus classified in the Parvoviridae family, known for its ability to effectively and securely transport genetic material into cells, making it valuable for gene therapy. Although industrial manufacturing methods for AAV vector production have advanced, challenges remain in terms of quality and production costs, hindering wider development.The limited scalability of current methods has resulted in prohibitively high costs for many patients seeking AAV gene therapy. Our hypothesis posits that natural AAV production in co-infected eukaryotic cells involves two distinct steps: capsid assembly and genetic material encapsidation.